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longer cycles raise copper-accumulation concern from GHK-Cu

7,9 However, as shown in Supplementary Figure, a couple of compounds with free thiol group and disulfide bond are present in the low-molecular-weight fraction of rat plasma, which can react with Ellmans reagent and provide apparent values for glutathione and its disulfide

Results Plasma processing condition significantly affects measured plasma levels of total GSH, free GSH, GSSG, and the ratio of free GSH to GSSG in plasma We found significant differences in total GSH levels (F 3,33 = 88.8411

We separated nuclear and cytoplasmic proteins and calculated the ratio of p-P53 to total P53 in each fraction

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